Marine Institute Open Access Repository: Recent submissions
Now showing items 21-40 of 1684
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Explorers Ocean Literacy Principle #5 The Ocean Supports a Great Diversity of Life and Ecosystems PresentationThe Explorers Ocean Literacy Principle #5 Presentation introduces you to the fifth principle of ocean literacy: the ocean supports a great diversity of life and ecosystems, and the concepts behind this principle. Slides can be used as a presentation or printed to display in the class. This PowerPoint may be used for educational purposes and the images must retain all of their image copyright detail
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Explorers Ocean Literacy Principle #4 The Ocean Makes Earth Habitable PresentationThe Explorers Ocean Literacy Principle #4 Presentation introduces you to the fourth principle of ocean literacy: the ocean makes Earth habitable, and the concepts behind this principle. Slides can be used as a presentation or printed to display in the class. This PowerPoint may be used for educational purposes and the images must retain all of their image copyright detail
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Explorers Ocean Literacy Principle #7 The Ocean is Largely Unexplored PresentationThe Explorers Ocean Literacy Principle #7 Presentation introduces you to the seventh principle of ocean literacy: the ocean is largely unexplored, and the concepts behind this principle. Slides can be used as a presentation or printed to display in the class. This PowerPoint may be used for educational purposes and the images must retain all of their image copyright detail
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Explorers Ocean Literacy Principle #3 The Ocean is a Major Influence on Weather and Climate PresentationThe Explorers Ocean Literacy Principle #3 Presentation introduces you to the third principle of ocean literacy: the ocean is a major influence on weather and climate, and the concepts behind this principle. Slides can be used as a presentation or printed to display in the class. This PowerPoint may be used for educational purposes and the images must retain all of their image copyright detail
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Explorers Ocean Literacy Principle #2 The Ocean and Life in the Ocean Shape the Features of Earth PresentationThe Explorers Ocean Literacy Principle #2 Presentation introduces you to the second principle of ocean literacy: the ocean and life in the ocean shape the features of Earth, and the concepts behind this principle. Slides can be used as a presentation or printed to display in the class. This PowerPoint may be used for educational purposes and the images must retain all of their image copyright detail
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Explorers Ocean Literacy Principle #1 Earth has One Big Ocean with Many Features PresentationThe Explorers Ocean Literacy Principle #1 Presentation introduces you to the first principle of ocean literacy: Earth has one big ocean with many features, and the concepts behind this principle. Slides can be used as a presentation or printed to display in the class. This PowerPoint may be used for educational purposes and the images must retain all of their image copyright detail
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Explorers Ocean Literacy Principles: What You Need to Know PresentationThis PowerPoint introduces the seven Ocean Literacy Principles, and is the first in a series going through each Principle in more detail. Slides can be used as a presentation or printed to display in the class. This PowerPoint may be used for educational purposes and the images must retain all of their image copyright details.
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Explorers Turtle Talk with Sea Turtles: Turtle Presentation Part 3: Turtle Special Features & Turtles in Irish WatersThe Explorers Turtle Talk with Sea Turtles: Turtle Presentation Part 3: Turtle Special Features & Turtles in Irish Waters introduces your class to the unique biology of turtles, from their life cycles to their migration prowess, plus the five turtle species that have been recorded in Irish waters. It is designed to accompany the Explorers Turtle Talk with Sea Turtles: An Introduction to Sea Turtles book. Slides can be used as a presentation or printed to display in the class. This PowerPoint may be used for educational purposes and the images must retain all of their image copyright details.
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Explorers Turtle Talk with Sea Turtles: Turtle Presentation Part 2: Turtles Stories & Turtle SurvivalThe Explorers Turtle Talk with Sea Turtles: Turtle Presentation Part 2: Turtles Stories & Turtle Survival introduces your class to the ways turtles have inspired stories from around the world and the conservation issues that affect their survival. It is designed to accompany the Explorers Turtle Talk with Sea Turtles: An Introduction to Sea Turtles book. Slides can be used as a presentation or printed to display in the class. This PowerPoint may be used for educational purposes and the images must retain all of their image copyright details.
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Explorers Turtle Talk with Sea Turtles: Turtle Presentation Part 1: Turtles, Extinction, and Flagship SpeciesThe Explorers Turtle Talk with Sea Turtles: Turtle Presentation Part 1: Turtles, Extinction, and Flagship Species introduces your class to the seven sea turtle species, why animals go extinct, and the concept of flagship species in conservation. It is designed to accompany the Explorers Turtle Talk with Sea Turtles: An Introduction to Sea Turtles book. Slides can be used as a presentation or printed to display in the class. This PowerPoint may be used for educational purposes and the images must retain all of their image copyright details.
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Explorers Sustainable Development Goals and the Ocean PresentationThis PowerPoint introduces the 17 Sustainable Development Goals (SDGs) and the ways in which they relate to each and to the ocean. Featuring photos, colourful graphics, fast facts, and lots of extra information in the notes including ideas for activities/discussion and further resources, this is an excellent way to introduce the SDGs to your class and discuss what they mean for us and our ocean. Slides can be used as a presentation or printed to display in the class. This PowerPoint may be used for educational purposes and the images must retain all of their image copyright details.
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Newport Research Facility, Annual Report No. 66, 2021This report represents a continuation of the scientific aspects of the Annual Reports published by the Salmon Research Agency of Ireland, now integrated into the Fisheries Ecosystem Advisory Services Group (FEAS) of the Marine Institute. The data presented creates a unique record of fish rearing and wild fish census data for the past 50 years. This data is an essential component in the local, regional and national management of salmon, sea trout and eel and is becoming ever more valuable in the light of increasing pressures on natural stocks, such as exploitation, habitat degradation and global climate change scenarios. The fish monitoring facilities in Newport, along with the reared and ranched salmon stocks held in Burrishoole, are also essential for supporting projects such as development of novel enhancement techniques, alternative stocks and ranching and evaluation of interactions between farmed, ranched and wild strains. An expanding programme in the Burrishoole system is including ecological and genetics research into eel, sticklebacks and stock dynamics of juvenile salmonids and eels.
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Explorers Turtle Talk with Sea Turtles: Teacher Planning Guide, Lesson Plans, and ActivitiesThe Explorers Turtle Talk with Sea Turtles: Teacher Planning Guide introduces teachers to the module and provides them with space to plan lessons for their class to accompany it
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Explorers Turtle Talk with Sea Turtles: My Sea Turtle Illustrations for design and creative activitiesThe Explorers Turtle Talk with Sea Turtles: My Sea Turtle Illustrations for design and creative activities is designed to accompany the activities in the My Turtle Talk Workbook, and the designs can be used as templates for activities like making a turtle tote bag or drawing a life-size turtle. The book can also be used as a colouring book for younger classes.
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Environmental Survey of Coastal Waters (Galway - Dublin – Galway): – Winter nutrients, benthic macro-invertebrate and contaminants monitoring 2022 (CV22-0013)The 2022 survey continues the Marine Institute’s Winter Nutrients monitoring that commenced in 1990/91. The survey has evolved and expanded during this time period with respect to target areas, parameters and sampling strategy. In 2011 this survey was re-established as a winter environmental survey with a broader remit to provide supporting information for OSPAR and Water Framework Directive (WFD- Directive 2000/60/EC) assessments and also to maintain the winter time series on key biogeochemical parameters in Irish waters in response to pressures such as land based inputs of nutrients and climate change. Since 2011 the survey circumnavigates the Island of Ireland every two years, alternating south-about and north-about, starting in the Irish Sea and ending in Galway. This provides a complete coverage of Ireland’s coastal waters over 2-year periods. However, given the timing of the surveys, winter by necessity to ensure minimal biological activity and therefore highest concentrations of dissolved nutrients, the weather is a significant factor in determining the actual as opposed to planned coverage of the target stations. The 2022 survey was designed to collect multidisciplinary information on physical conditions, water chemistry (dissolved nutrients, total alkalinity (TA), dissolved inorganic carbon (DIC) and salinity), sediment chemistry (persistent organic pollutants POPs and trace metals), sediment particle size distribution and benthic macroinvertebrates (at targeted waterbodies around the coast). This contributes to data collection needs of various statutory drivers (WFD and the Marine Strategy Framework Directive (MSFD) Directive 2008/56/EC) as well as providing a research dataset on status and changing conditions (trends and variations) for key environmental variables. As a result of the COVID pandemic, in 2021 operational adjustments were implemented such that scientific complement on-board was limited to 2-3 persons at any one time. In light of this, the survey plan was adjusted to allow the survey to be completed in two legs (both south-about): 1. Leg 1 – Galway – Dublin: benthic macro-invertebrate sampling 2. Leg 2 – Dublin – Galway: winter nutrient, carbon and contaminants sampling. This survey structure was continued in 2022. Furthermore, and in order to fully achieve this plan, the number of survey days was increased to 17.
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Epimers of Azaspiracids: Isolation, Structural Elucidation, Relative LC-MS Response, and in Vitro Toxicity of 37-epi-Azaspiracid-1Since azaspiracid-1 (AZA1) was identified in 1998, the number of AZA analogues has increased to over 30. The development of an LC-MS method using a neutral mobile phase led to the discovery of isomers of AZA1, AZA2, and AZA3, present at ∼2–16% of the parent analogues in phytoplankton and shellfish samples. Under acidic mobile phase conditions, isomers and their parents are not separated. Stability studies showed that these isomers were spontaneous epimerization products whose formation is accelerated with the application of heat. The AZA1 isomer was isolated from contaminated shellfish and identified as 37-epi-AZA1 by nuclear magnetic resonance (NMR) spectroscopy and chemical analyses. Similar analysis indicated that the isomers of AZA2 and AZA3 corresponded to 37-epi-AZA2 and 37-epi-AZA3, respectively. The 37-epimers were found to exist in equilibrium with the parent compounds in solution. 37-epi-AZA1 was quantitated by NMR, and relative molar response studies were performed to determine the potential differences in LC-MS response of AZA1 and 37-epi-AZA1. Toxicological effects were determined using Jurkat T lymphocyte cells as an in vitro cell model. Cytotoxicity experiments employing a metabolically based dye (i.e., MTS) indicated that 37-epi-AZA1 elicited a lethal response that was both concentration- and time-dependent, with EC50 values in the subnanomolar range. On the basis of EC50 comparisons, 37-epi-AZA1 was 5.1-fold more potent than AZA1. This data suggests that the presence of these epimers in seafood products should be considered in the analysis of AZAs for regulatory purposes.
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Structure Elucidation, Relative LC–MS Response and In Vitro Toxicity of Azaspiracids Isolated from Mussels (Mytilus edulis)Azaspiracids (AZAs) are marine biotoxins produced by dinoflagellates that can accumulate in shellfish, which if consumed can lead to poisoning events. AZA7–10, 7–10, were isolated from shellfish and their structures, previously proposed on the basis of only LC–MS/MS data, were confirmed by NMR spectroscopy. Purified AZA4–6, 4–6, and 7–10 were accurately quantitated by qNMR and used to assay cytotoxicity with Jurkat T lymphocyte cells for the first time. LC–MS(MS) molar response studies performed using isocratic and gradient elution in both selected ion monitoring and selected reaction monitoring modes showed that responses for the analogues ranged from 0.3 to 1.2 relative to AZA1, 1. All AZA analogues tested were cytotoxic to Jurkat T lymphocyte cells in a time- and concentration-dependent manner; however, there were distinct differences in their EC50 values, with the potencies for each analogue being: AZA6 > AZA8 > AZA1 > AZA4 ≈ AZA9 > AZA5 ≈ AZA10. This data contributes to the understanding of the structure–activity relationships of AZAs.
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Identification of 21,22-Dehydroazaspiracids in Mussels (Mytilus edulis) and in Vitro Toxicity of Azaspiracid-26Azaspiracids (AZAs) are marine biotoxins produced by the genera Azadinium and Amphidoma, pelagic marine dinoflagellates that may accumulate in shellfish resulting in human illness following consumption. The complexity of these toxins has been well documented, with more than 40 structural variants reported that are produced by dinoflagellates, result from metabolism in shellfish, or are extraction artifacts. Approximately 34 μg of a new AZA with MW 823 Da (AZA26 (3)) was isolated from blue mussels (Mytilus edulis), and its structure determined by MS and NMR spectroscopy. AZA26, possibly a bioconversion product of AZA5, lacked the C-20–C-21 diol present in all AZAs reported thus far and had a 21,22-olefin and a keto group at C-23. Toxicological assessment of 3 using an in vitro model system based on Jurkat T lymphocyte cells showed the potency to be ∼30-fold lower than that of AZA1. The corresponding 21,22-dehydro-23-oxo-analogue of AZA10 (AZA28) and 21,22-dehydro analogues of AZA3, -4, -5, -6, -9, and -10 (AZA25, -48 (4), -60, -27, -49, and -61, respectively) were also identified by HRMS/MS, periodate cleavage reactivity, conversion from known analogues, and NMR (for 4 that was present in a partially purified sample of AZA7).
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Ocean Superhero Comic Book TemplateTemplate to create a comic about an ocean superhero that tackles climate change. This can be used to complement the other lessons on an ocean climate superhero.
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Population structure and connectivity in the genus Molva in the Northeast AtlanticIn fisheries, operational management units and biological data often do not coincide. In many cases, this is not even known due to the lack of information about a species’ population structure or behaviour. This study focuses on two such species, the common ling Molva molva and the blue ling M. dypterygia, two Northeast Atlantic gadoids with overlapping geographical distribution, but different depth habitats. Heavily exploited throughout their ranges, with declining catches, little is known about their population structure. Genotyping-by-sequencing at thousands of genetic markers indicated that both species are separated into two major groups, one represented by samples from the coasts of western Scotland, Greenland, and the Bay of Biscay and the other off the coast of Norway. This signal is stronger for the deeper dwelling blue ling, even though adult dispersal was also identified for this species. Despite small sample sizes, fine-scale patterns of genetic structure were identified along Norway for common ling. Signatures of adaptation in blue ling consisted in signs of selections in genes involved in vision, growth, and adaptation to cold temperatures.
