• Login
    View Item 
    •   Marine Institute Open Access Repository
    • Marine Institute Community of Research Publications
    • Marine Environment & Food Safety Services
    • Shellfish Microbiology
    • View Item
    •   Marine Institute Open Access Repository
    • Marine Institute Community of Research Publications
    • Marine Environment & Food Safety Services
    • Shellfish Microbiology
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of Marine OARCommunitiesPublication DateAuthorsTitlesSubjectsThis CollectionPublication DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    Display statistics

    Characterisation of norovirus contamination in an Irish shellfishery using real-time RT-qPCR and sequencing analysis

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Rajko-Nenow et al Characterisation ...
    Size:
    204.5Kb
    Format:
    PDF
    Download
    Author
    Rajko-Nenow, Paulina
    Keaveney, Sinéad
    Flannery, John
    O'Flaherty, Vincent
    Doré, William
    Keyword
    Norovirus
    Oysters
    Seasonality
    Multiple genotypes
    RT-qPCR
    Date
    2012
    Publisher
    Elsevier
    
    Metadata
    Show full item record
    Abstract
    Norovirus (NoV) is the single most important agent of foodborne viral gastroenteritis worldwide. Bivalve shellfish, such as oysters, grown in areas contaminated with human faecal waste may become contaminated with human pathogens including NoV. A study was undertaken to investigate NoV contamination in oysters (Crassostrea gigas) from a shellfishery over a 24 month period from October 2007 to September 2009. Oyster samples were collected monthly from a commercial shellfish harvest area classified as category B under EU regulations, but that had had been closed for commercial harvesting due to its previous association with NoV outbreaks. Real-time reverse transcription quantitative PCR (RT-qPCR) was used to determine the concentration of human NoV genogroups I and II (GI and GII) in monthly samples. Total NoV (GI and GII) concentrations in NoV positive oysters ranged from 97 to 20,080 genome copies g− 1 of digestive tissue and displayed a strong seasonal trend with greater concentrations occurring during the winter months. While NoV GII concentrations detected in oysters during both years were similar, NoV GI concentrations were significantly greater in oysters during the winter of 2008/09 than during the winter of 2007/08. To examine the NoV genotypes present in oyster samples, sequence analysis of nested RT-PCR products was undertaken. Although NoV GII.4 is responsible for the vast majority of reports of outbreaks in the community, multiple NoV genotypes were identified in oysters during this study: GI.4, GI.3, GI.2, GII.4, GII.b, GII.2, GII.12, and GII.e. NoV GI.4 was the most frequently detected genotype throughout the study period and was detected in 88.9% of positive samples, this was followed by GII.4 (43.7%) and GII.b (37.5%). This data demonstrates the diversity of NoV genotypes that can be present in sewage contaminated shellfish and that a disproportionate number of non-NoV GII.4 genotypes can be found in environmental samples compared to the number of recorded human infections associated with non-NoV GII.4 genotypes.
    URI
    http://hdl.handle.net/10793/862
    Description
    Copyright © 2012 Rajko-Nenow et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Peer reviewed
    Citation
    Rajko-Nenow, P., Keaveney, S., Flannery, J., O’Flaherty, V., & Doré, W. (2012). Characterisation of norovirus contamination in an Irish shellfishery using real-time RT-qPCR and sequencing analysis. International Journal of Food Microbiology, 160(2), 105–112. doi:10.1016/j.ijfoodmicro.2012.10.001
    Collections
    Shellfish Microbiology

    entitlement

     
    DSpace software (copyright © 2002 - 2025)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.