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dc.contributor.authorJauffrais, Thierry
dc.contributor.authorKilcoyne, Jane
dc.contributor.authorSéchet, Véronique
dc.contributor.authorHerrenknecht, Christine
dc.contributor.authorTruquet, Philippe
dc.contributor.authorHervé, Fabienne
dc.contributor.authorBérard, Jean Baptiste
dc.contributor.authorNulty, Cíara
dc.contributor.authorTaylor, Sarah
dc.contributor.authorTillmann, Urban
dc.contributor.authorMiles, Christopher O.
dc.contributor.authorHess, Philipp
dc.date.accessioned2012-08-02T12:57:00Z
dc.date.available2012-08-02T12:57:00Z
dc.date.issued2012
dc.identifier.citationJauffrais T., Kilcoyne J., Séchet V., Herrenknecht C., Truquet P., Hervé F., Bérard J.B., Nulty C., Taylor S., Tillmann U., Miles C.O., & Hess P. (2012). Production and Isolation of Azaspiracid-1 and -2 from Azadinium spinosum Culture in Pilot Scale Photobioreactors. Marine Drugs, 10(6), 1360-1382.en_GB
dc.identifier.issn1660-3397
dc.identifier.urihttp://hdl.handle.net/10793/806
dc.identifier.urihttp://dx.doi.org/10.3390/md10061360
dc.descriptionThis article was originally published in Marine Drugs and can be accessed at http://dx.doi.org/10.3390/md10061360.en_GB
dc.descriptionpeer-reviewed
dc.description.abstractAzaspiracid (AZA) poisoning has been reported following consumption of contaminated shellfish, and is of human health concern. Hence, it is important to have sustainable amounts of the causative toxins available for toxicological studies and for instrument calibration in monitoring programs, without having to rely on natural toxin events. Continuous pilot scale culturing was carried out to evaluate the feasibility of AZA production using Azadinium spinosum cultures. Algae were harvested using tangential flow filtration or continuous centrifugation. AZAs were extracted using solid phase extraction (SPE) procedures, and subsequently purified. When coupling two stirred photobioreactors in series, cell concentrations reached 190,000 and 210,000 cell•mL−1 at steady state in bioreactors 1 and 2, respectively. The AZA cell quota decreased as the dilution rate increased from 0.15 to 0.3 day−1, with optimum toxin production at 0.25 day−1. After optimization, SPE procedures allowed for the recovery of 79 ± 9% of AZAs. The preparative isolation procedure previously developed for shellfish was optimized for algal extracts, such that only four steps were necessary to obtain purified AZA1 and -2. A purification efficiency of more than 70% was achieved, and isolation from 1200 L of culture yielded 9.3 mg of AZA1 and 2.2 mg of AZA2 of >95% purity. This work demonstrated the feasibility of sustainably producing AZA1 and -2 from A. spinosum cultures.en_GB
dc.language.isoenen_GB
dc.publisherMPDI Publishingen_GB
dc.relation.ispartofseriesMarine Drugs;10 (6)
dc.subjectSolid phase extractionen_GB
dc.subjectPhotobioreactoren_GB
dc.subjectChemostaten_GB
dc.subjectDinoflagellateen_GB
dc.subjectMicro-algaeen_GB
dc.subjectLC-MS/MSen_GB
dc.subjectTangential flow filtrationen_GB
dc.subjectAzaspiraciden_GB
dc.subjectHP-20en_GB
dc.titleProduction and Isolation of Azaspiracid-1 and -2 from Azadinium spinosum Culture in Pilot Scale Photobioreactorsen_GB
dc.typeArticleen_GB
refterms.dateFOA2018-01-12T04:51:38Z


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