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dc.contributor.authorHess, P.
dc.contributor.authorMorris, S.
dc.contributor.authorStobo, L.A.
dc.contributor.authorBrown, N.A.
dc.contributor.authorMcEvoy, J.D.G.
dc.contributor.authorKennedy, G.
dc.contributor.authorYoung, P.B.
dc.contributor.authorSlattery, D.
dc.contributor.authorMcGovern, E.
dc.contributor.authorMcMahon, T.
dc.contributor.authorGallacher, S.
dc.date.accessioned2017-04-06T11:25:12Z
dc.date.available2017-04-06T11:25:12Z
dc.date.issued2005
dc.identifier.citationHess, P., Morris, S., Stobo, L.A., Brown, N.A., McEvoy, J.D.G., Kennedy, G., Young, P.B., Slattery, D., McGovern, E., McMahon, T. and Gallacher, S. (2005). LC-UV and LC-MS methods for the determination of domoic acid. Trends in Analytical Chemistry, 24(4), pp. 358–367 http://dx.doi.org/10.1016/j.trac.2004.11.019en_GB
dc.identifier.issn0165-9936
dc.identifier.urihttp://hdl.handle.net/10793/1280
dc.descriptionPeer-reviewed. Released with a Creative Commons Attribution Non-Commercial No Derivatives Licenseen_GB
dc.description.abstractUnder European legislation, domoic acid (DA), the main constituent of amnesic shellfish poisoning, is monitored to protect the shellfish consumer. To ensure comparability amongst analytical data, it was deemed necessary to undertake performance assessments of the methods conducted by monitoring laboratories of the United Kingdom and Ireland. In phase I of a two-phase inter-comparison, three laboratories used high-performance liquid chromatography and ultraviolet detection (HPLC-UV). Concentration data for a DA standard solution, a crude extract of whole scallops and a scallop-homogenate fell within internationally accepted limits, demonstrating good agreement for these matrices. Between-laboratory analyses of a scallop gonad showed a higher variation (>16%). In phase II, a second gonad homogenate containing DA one order of magnitude higher in concentration gave results acceptable to internationally set criteria. The efficiency of the strong anion-exchange cartridges used in sample-extract clean-up should be monitored as part of a laboratory quality control system. From a recovery study, it is suggested that recovery correction should also be applied. There was no difference in the quantitation of DA in standard solutions or shellfish using either LC-UV or LC with mass spectrometric (MS) detection, and between-laboratory MS data for a gonad homogenate were also equivalent. Variations of the published method practised by the monitoring laboratories were found not to compromise results, thus demonstrating an acceptable degree of ruggedness, as well as comparability between the participants.en_GB
dc.description.sponsorshipUK Food Standards Agency, and the Scottish Environment Executive Rural Affairs Department.en_GB
dc.language.isoenen_GB
dc.publisherElsevieren_GB
dc.relation.ispartofseriesTrends in analytical chemistry;24(4)
dc.subjectDomoic aciden_GB
dc.subjectLC-MSen_GB
dc.subjectLC-UVen_GB
dc.subjectRuggednessen_GB
dc.subjectScallopsen_GB
dc.subjectStandard solutionen_GB
dc.titleLC-UV and LC-MS methods for the determination of domoic aciden_GB
dc.typeArticleen_GB
refterms.dateFOA2018-01-12T06:09:30Z


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