Show simple item record

dc.contributor.authorRajow-Nenow, P.
dc.contributor.authorWaters, A.
dc.contributor.authorKeaveney, S.
dc.contributor.authorFlannery, J.
dc.contributor.authorTuite, G.
dc.contributor.authorCoughlan, S.
dc.contributor.authorO’Flaherty, V.
dc.contributor.authorDoré, W.
dc.date.accessioned2017-03-23T12:17:12Z
dc.date.available2017-03-23T12:17:12Z
dc.date.issued2013
dc.identifier.citationRajko-Nenowa, P., Waters, A., Keaveney, S., Flannery, J., Tuite, G., Coughlan, S., O'Flaherty, V. and Doré, W. (2013. Norovirus genotypes present in oysters and in effluent from a wastewater treatment plant during the seasonal peak of infections in Ireland in 2010. Applied and environmental microbiology, 79(8), pp. 2578–2587 doi:10.1128/AEM.03557-12en_GB
dc.identifier.issn0099-2240
dc.identifier.urihttp://hdl.handle.net/10793/1259
dc.descriptionPeer-reviewed.en_GB
dc.description.abstractWe determined norovirus (NoV) concentrations in effluent from a wastewater treatment plant and in oysters during the peak period of laboratory-confirmed cases of NoV infection in Ireland in 2010 (January to March). Weekly samples of influent, secondary treated effluent, and oysters were analyzed using real-time quantitative reverse transcription-PCR for NoV genogroup I (GI) and genogroup II (GII). The mean concentration of NoV GII (5.87 104 genome copies 100 ml 1) in influent wastewater was significantly higher than the mean concentration of NoV GI (1.40 104 genome copies 100 ml 1). The highest concentration of NoV GII (2.20 105 genome copies 100 ml 1) was detected in influent wastewater during week 6. Over the study period, a total of 931 laboratory-confirmed cases of NoV GII infection were recorded, with the peak (n 171) occurring in week 7. In comparison, 16 cases of NoV GI-associated illness were reported during the study period. In addition, the NoV capsid N/S domain was molecularly characterized for selected samples. Multiple genotypes of NoV GI (GI.1, GI.4, GI.5, GI.6, and GI.7) and GII (GII.3, GII.4, GII.6, GII.7, GII.12, GII.13, and GII.17), as well as 4 putative recombinant strains, were detected in the environmental samples. The NoV GII.4 variant 2010 was detected in wastewater and oyster samples and was the dominant strain detected in NoV outbreaks at that time. This study demonstrates the diversity of NoV genotypes present in wastewater during a period of high rates of NoV infection in the community and highlights the potential for the environmental spread of multiple NoV genotypes.en_GB
dc.language.isoenen_GB
dc.publisherAmerican Society for Microbiologyen_GB
dc.relation.ispartofseriesApplied and environmental microbiology;79(8)
dc.subjectNorovirusen_GB
dc.subjectOysteren_GB
dc.subjectWastewater treatmenten_GB
dc.subjectIrelanden_GB
dc.titleNorovirus genotypes present in oysters and in effluent from a wastewater treatment plant during the seasonal peak of infections in Ireland in 2010en_GB
dc.typeArticleen_GB
refterms.dateFOA2018-01-12T06:08:27Z


Files in this item

Thumbnail
Name:
Rajko-Nenow et al. Norovirus ...
Size:
1.414Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record