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dc.contributor.authorDonohoe, O. H.
dc.contributor.authorHenshilwood, K.
dc.contributor.authorWay, K.
dc.contributor.authorHakimjavadi, R.
dc.contributor.authorStone, D. M.
dc.contributor.authorWalls, D.
dc.date.accessioned2015-05-07T10:48:23Z
dc.date.available2015-05-07T10:48:23Z
dc.date.issued2015
dc.identifier.citationDonohoe OH, Henshilwood K, Way K, Hakimjavadi R, Stone DM, Walls D (2015) Identification and Characterization of Cyprinid Herpesvirus-3 (CyHV-3) Encoded MicroRNAs. PLoS ONE 10(4): e0125434. doi:10.1371/journal.pone.0125434en_GB
dc.identifier.other•DOI: 10.1371/journal.pone.0125434
dc.identifier.urihttp://hdl.handle.net/10793/1081
dc.descriptionData Availability: Relevant data are contained within the paper and its Supporting Information files. All unique sequence tags obtained following virus infections are available for download via the Dryad Digital Repository (http://dx.doi.org/10.5061/dryad.3c7m4).en_GB
dc.description.abstractMicroRNAs (miRNAs) are a class of small non-coding RNAs involved in post-transcriptional gene regulation. Some viruses encode their own miRNAs and these are increasingly being recognized as important modulators of viral and host gene expression. Cyprinid herpesvirus 3 (CyHV-3) is a highly pathogenic agent that causes acute mass mortalities in carp (Cyprinus carpio carpio) and koi (Cyprinus carpio koi) worldwide. Here, bioinformatic analyses of the CyHV-3 genome suggested the presence of non-conserved precursor miRNA (pre-miRNA) genes. Deep sequencing of small RNA fractions prepared from in vitro CyHV-3 infections led to the identification of potential miRNAs and miRNA–offset RNAs (moRNAs) derived from some bioinformatically predicted pre-miRNAs. DNA microarray hybridization analysis, Northern blotting and stem-loop RT-qPCR were then used to definitively confirm that CyHV-3 expresses two pre-miRNAs during infection in vitro. The evidence also suggested the presence of an additional four high-probability and two putative viral pre-miRNAs. MiRNAs from the two confirmed pre-miRNAs were also detected in gill tissue from CyHV-3-infected carp. We also present evidence that one confirmed miRNA can regulate the expression of a putative CyHV-3-encoded dUTPase. Candidate homologues of some CyHV-3 pre-miRNAs were identified in CyHV-1 and CyHV-2. This is the first report of miRNA and moRNA genes encoded by members of the Alloherpesviridae family, a group distantly related to the Herpesviridae family. The discovery of these novel CyHV-3 genes may help further our understanding of the biology of this economically important virus and their encoded miRNAs may have potential as biomarkers for the diagnosis of latent CyHV-3.en_GB
dc.description.sponsorshipFunding: This work was supported by the Marine Institute Research Fellowship programme (https://www.marine.ie; OHD), Defra https://www.gov.uk/government/organisati​ons/department-for-environment-food-rura​l-affairs; research contracts FC1193 and FC1202; KW), the Programme for Research in Third Level Institutions (PRTLI) (http://www.hea.ie/en/prtli,official; RH, DW) Cycle 5 [The Bioanalysis and Therapeutics (BioAT) programme at DCU]. The PRTLI is co-funded through the European Regional Development Fund (ERDF), part of the European Union Structural Funds Programme 2007–2013 (http://eustructuralfunds.gov.ie). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en_GB
dc.language.isoenen_GB
dc.publisherPLoS ONEen_GB
dc.relation.ispartofseriesPLoS ONE;
dc.subjectMicroRNAs (miRNAs)en_GB
dc.subjectCyprinid herpesvirus 3 (CyHV-3)en_GB
dc.subjectgene regulationen_GB
dc.subjectvirusesen_GB
dc.subjectcarp (Cyprinus carpio carpio)en_GB
dc.subjectkoi (Cyprinus carpio koi)en_GB
dc.subjectRNAen_GB
dc.subjectmiRNA–offset RNAs (moRNAs)en_GB
dc.titleIdentification and Characterization of Cyprinid Herpesvirus-3 (CyHV-3) Encoded MicroRNAsen_GB
dc.typeArticleen_GB
refterms.dateFOA2018-01-12T03:04:27Z


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