Critical Role of the Matricellular Protein SPARC in Mediating Erythroid Progenitor Cell Development in Zebrafish

Sparc (Osteonectin) is a multifunctional matricellular glycoprotein expressed by many differentiated cells. Members of this family mediate cell-matrix interactions rather than acting as structural components of the extracellular matrix and therefore can influence many remodelling events, including haematopoiesis. We have investigated the role of sparc in embryonic haematopoiesis, using a morpholino antisense oligonucleotidebased knockdown approach. Knockdown of sparc function resulted in specific erythroid progenitor cell differentiation defects that were highlighted by changes in gene expression and morphology, which could be rescued by injection of sparc mRNA. Furthermore, a comparison of blood phenotypes of sparc and fgfs knockdowns with similar defects and the sparc rescue of fgf21 blood phenotype places sparc downstream of fgf21 in the genetic network regulating haematopoiesis in zebrafish. These results establish a role for an extracellular matrix protein (Sparc) as an important regulator of embryonic haematopoiesis during early development in zebrafish.

URI

http://hdl.handle.net/10793/846

Description

NOTICE: this is the author’s version of a work that was accepted for publication in Cells Tissues Organs. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. The Copyright for this material belongs to Karger. A definitive version was subsequently Cells Tissues Organs, [Issue 197, (November 2012)] (DOI: 10.1159/000343291), http://content.karger.com/ProdukteDB/produkte.asp?Doi=343291
peer-reviewed

Citation

Ceinos RM, Torres-Nuñez E, Chamorro R, Novoa B, Figueras A, Ruane NM, Rotllant J: Critical Role of the Matricellular Protein SPARC in Mediating Erythroid Progenitor Cell Development in Zebrafish. Cells Tissues Organs 2013;197:196-208 (DOI: 10.1159/000343291)