Marine Institute Open Access Repository >
Marine Institute Community of Research Publications >
Marine Environment & Food Safety Services >
Fish Health >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10793/94

Title: Molecular differentiation of infectious pancreatic necrosis virus isolates from farmed and wild salmonids in Ireland
Authors: McCarthy, L
Swords, D
Ruane, N. M.
Keywords: epidemiology
infectious pancreatic necrosis virus
phylogeny
VP2 gene
Issue Date: 2009
Publisher: Wiley-Blackwell
Citation: Ruane, N. M., McCarthy, L. J., Swords, D. and Henshilwood, K. (2009), Molecular differentiation of infectious pancreatic necrosis virus isolates from farmed and wild salmonids in Ireland. Journal of Fish Diseases, 32: 979–987. doi: 10.1111/j.1365-2761.2009.01080.x
Series/Report no.: Journal of Fish Diseases;32(12)
Abstract: This study investigated the genotypes and sub-groups of infectious pancreatic necrosis virus (IPNV) present in farmed and wild salmonid fish in Ireland. An 1100-bp portion of the VP2 region of segment A from each of 55 IPNV isolates collected over 2003–2007 was amplified by reverse-transcription–polymerase chain reaction and the product directly sequenced. The nucleotide sequences of each isolate were aligned and compared with each other and with the corresponding sequences of a number of reference isolates. All the 55 sequenced isolates belonged to genogroup 5 (Sp serotype) and could be divided into two subgroups. Irish subgroup 1 consisted of isolates from farmed salmon originating from an Irish salmon broodstock. Irish subgroup 2 consisted of isolates from imported farmed stock and all reported clinical outbreaks of IPN were associated with isolates from subgroup 2. Isolates from wild fish were identical to some isolates from subgroup 2, and therefore are believed to have originated from infected farms. These results highlight the importance of import risk analysis for diseases not listed under current legislation.
Description: peer-reviewed
URI: http://hdl.handle.net/10793/94
ISSN: 1365-2761
Appears in Collections:Fish Health

Files in This Item:

File Description SizeFormat
Molecular differentiation of infectious pancreatic necrosis virus isolates from farmed and wild salmonids in Ireland repository.pdf68.24 kBAdobe PDFView/Open
Use License

Items in the Marine Institute Open Access Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! Marine Institute Copyright © 2011  - Feedback