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Please use this identifier to cite or link to this item: http://hdl.handle.net/10793/806

Title: Production and Isolation of Azaspiracid-1 and -2 from Azadinium spinosum Culture in Pilot Scale Photobioreactors
Authors: Jauffrais, Thierry
Kilcoyne, Jane
Séchet, Véronique
Herrenknecht, Christine
Truquet, Philippe
Hervé, Fabienne
Bérard, Jean Baptiste
Nulty, Cíara
Taylor, Sarah
Tillmann, Urban
Miles, Christopher O.
Hess, Philipp
Keywords: Solid phase extraction
Photobioreactor
Chemostat
Dinoflagellate
Micro-algae
LC-MS/MS
Tangential flow filtration
Azaspiracid
HP-20
Issue Date: 2012
Publisher: MPDI Publishing
Citation: Jauffrais T., Kilcoyne J., Séchet V., Herrenknecht C., Truquet P., Hervé F., Bérard J.B., Nulty C., Taylor S., Tillmann U., Miles C.O., & Hess P. (2012). Production and Isolation of Azaspiracid-1 and -2 from Azadinium spinosum Culture in Pilot Scale Photobioreactors. Marine Drugs, 10(6), 1360-1382.
Series/Report no.: Marine Drugs;10 (6)
Abstract: Azaspiracid (AZA) poisoning has been reported following consumption of contaminated shellfish, and is of human health concern. Hence, it is important to have sustainable amounts of the causative toxins available for toxicological studies and for instrument calibration in monitoring programs, without having to rely on natural toxin events. Continuous pilot scale culturing was carried out to evaluate the feasibility of AZA production using Azadinium spinosum cultures. Algae were harvested using tangential flow filtration or continuous centrifugation. AZAs were extracted using solid phase extraction (SPE) procedures, and subsequently purified. When coupling two stirred photobioreactors in series, cell concentrations reached 190,000 and 210,000 cell•mL−1 at steady state in bioreactors 1 and 2, respectively. The AZA cell quota decreased as the dilution rate increased from 0.15 to 0.3 day−1, with optimum toxin production at 0.25 day−1. After optimization, SPE procedures allowed for the recovery of 79 ± 9% of AZAs. The preparative isolation procedure previously developed for shellfish was optimized for algal extracts, such that only four steps were necessary to obtain purified AZA1 and -2. A purification efficiency of more than 70% was achieved, and isolation from 1200 L of culture yielded 9.3 mg of AZA1 and 2.2 mg of AZA2 of >95% purity. This work demonstrated the feasibility of sustainably producing AZA1 and -2 from A. spinosum cultures.
Description: This article was originally published in Marine Drugs and can be accessed at http://dx.doi.org/10.3390/md10061360.
peer-reviewed
URI: http://hdl.handle.net/10793/806
http://dx.doi.org/10.3390/md10061360
ISSN: 1660-3397
Appears in Collections:Peer Reviewed Scientific Papers

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