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Please use this identifier to cite or link to this item: http://hdl.handle.net/10793/424

Title: Strategies for the elimination of matrix effects in the LC-MS/MS analysis of the lipophilic toxins okadaic acid and azaspiracid-1 in molluscan shellfish
Authors: Kilcoyne, J
Fux, E
Keywords: Lipophilic marine biotoxins
Azaspiracid
Okadaic acid,
LC–MS
On-line SPE
Matrix Effects
Issue Date: 2010
Publisher: Elsevier
Citation: Jane Kilcoyne, Elie Fux, Strategies for the elimination of matrix effects in the liquid chromatography tandem mass spectrometry analysis of the lipophilic toxins okadaic acid and azaspiracid-1 in molluscan shellfish, Journal of Chromatography A, Volume 1217, Issue 45, 5 November 2010, Pages 7123-7130, ISSN 0021-9673, DOI: 10.1016/j.chroma.2010.09.020
Series/Report no.: Journal of Chromatography A;1217 (45)
Abstract: Considerable efforts are being made worldwide to replace in vivo assays with instrumental methods of analysis for the monitoring of marine biotoxins in shellfish. Analysis of these compounds by the preferred technique of LC-MS/MS is challenged by matrix effects associated with shellfish tissue components. In methods validation, assessment of matrix interferences is imperative to ensure the accuracy of analytical results. We evaluated matrix interferences in the analysis of okadaic acid (OA) and azaspiracid 1 (AZA1) in mollucscan shellfish by using a conventional acidic method on electrospray triple stage quadrapole (TSQ) and hybrid quadrupole time of flight (QToF) instruments, with matrix matched standards for several species. Using the acidic method, we found no matrix interferences for OA, and matrix suppression for AZA1, with the TSQ instrument; in contrast, we found matrix enhancement for OA, and no matrix interference for AZA1, with QToF. The suppression of AZA1 signal on the TSQ instrument was due to interfering compounds carried over from previous injections. The degree of suppression was dependent on the tissue type, ranging from 20 to 70%. Several strategies were evaluated to eliminate these interferences, including the partitioning of the extract with hexane, optimization of the chromatographic
Description: NOTICE: this is the author’s version of a work that was accepted for publication in Journal of Chromatography A. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Chromatography A, [Volume 1217, Issue 45 (5 November 2010)] doi:10.1016/j.chroma.2010.09.020 http://www.sciencedirect.com/science/article/pii/S0021967310012409
peer-reviewed
URI: http://hdl.handle.net/10793/424
http://dx.doi.org/10.1016/j.chroma.2010.09.020
ISSN: 0021-9673
Appears in Collections:Peer Reviewed Scientific Papers

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