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Please use this identifier to cite or link to this item: http://hdl.handle.net/10793/1265

Title: New insights into the causes of human illness due to consumption of azaspiracid contaminated shellfish
Authors: Chevallier, O.P.
Graham, S.F.
Alonso, E.
Duffy, C.
Silke, J.
Campbell, K.
Botana, L.M.
Elliott, C.T.
Keywords: Azaspiracid
Shellfish
Issue Date: 2015
Publisher: Nature Publishing Group
Citation: Chevallier, O.P., Graham, S.F., Alonso, E., Duffy, C., Silke, J., Campbell, K., Botana, L.M. and Elliott, C.T. (2015). New insights into the causes of human illness due to consumption of azaspiracid contaminated shellfish. Scientific reports, 5(9818) DOI:10.1038/srep09818
Series/Report no.: Scientific reports;5(9818)
Abstract: Azaspiracid (AZA) poisoning was unknown until 1995 when shellfish harvested in Ireland caused illness manifesting by vomiting and diarrhoea. Further in vivo/vitro studies showed neurotoxicity linked with AZA exposure. However, the biological target of the toxin which will help explain such potent neurological activity is still unknown. A region of Irish coastline was selected and shellfish were sampled and tested for AZA using mass spectrometry. An outbreak was identified in 2010 and samples collected before and after the contamination episode were compared for their metabolite profile using high resolution mass spectrometry. Twenty eight ions were identified at higher concentration in the contaminated samples. Stringent bioinformatic analysis revealed putative identifications for seven compounds including, glutarylcarnitine, a glutaric acid metabolite. Glutaric acid, the parent compound linked with human neurological manifestations was subjected to toxicological investigations but was found to have no specific effect on the sodium channel (as was the case with AZA). However in combination, glutaric acid (1mM) and azaspiracid (50nM) inhibited the activity of the sodium channel by over 50%. Glutaric acid was subsequently detected in all shellfish employed in the study. For the first time a viable mechanism for how AZA manifests itself as a toxin is presented.
Description: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material.
URI: http://hdl.handle.net/10793/1265
ISSN: 2045-2322
Appears in Collections:Shellfish Safety

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