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Please use this identifier to cite or link to this item: http://hdl.handle.net/10793/1081

Title: Identification and Characterization of Cyprinid Herpesvirus-3 (CyHV-3) Encoded MicroRNAs
Authors: Donohoe, O. H.
Henshilwood, K.
Way, K.
Hakimjavadi, R.
Stone, D. M.
Walls, D.
Keywords: MicroRNAs (miRNAs)
Cyprinid herpesvirus 3 (CyHV-3)
gene regulation
viruses
carp (Cyprinus carpio carpio)
koi (Cyprinus carpio koi)
RNA
miRNA–offset RNAs (moRNAs)
Issue Date: 2015
Publisher: PLoS ONE
Citation: Donohoe OH, Henshilwood K, Way K, Hakimjavadi R, Stone DM, Walls D (2015) Identification and Characterization of Cyprinid Herpesvirus-3 (CyHV-3) Encoded MicroRNAs. PLoS ONE 10(4): e0125434. doi:10.1371/journal.pone.0125434
Series/Report no.: PLoS ONE;
Abstract: MicroRNAs (miRNAs) are a class of small non-coding RNAs involved in post-transcriptional gene regulation. Some viruses encode their own miRNAs and these are increasingly being recognized as important modulators of viral and host gene expression. Cyprinid herpesvirus 3 (CyHV-3) is a highly pathogenic agent that causes acute mass mortalities in carp (Cyprinus carpio carpio) and koi (Cyprinus carpio koi) worldwide. Here, bioinformatic analyses of the CyHV-3 genome suggested the presence of non-conserved precursor miRNA (pre-miRNA) genes. Deep sequencing of small RNA fractions prepared from in vitro CyHV-3 infections led to the identification of potential miRNAs and miRNA–offset RNAs (moRNAs) derived from some bioinformatically predicted pre-miRNAs. DNA microarray hybridization analysis, Northern blotting and stem-loop RT-qPCR were then used to definitively confirm that CyHV-3 expresses two pre-miRNAs during infection in vitro. The evidence also suggested the presence of an additional four high-probability and two putative viral pre-miRNAs. MiRNAs from the two confirmed pre-miRNAs were also detected in gill tissue from CyHV-3-infected carp. We also present evidence that one confirmed miRNA can regulate the expression of a putative CyHV-3-encoded dUTPase. Candidate homologues of some CyHV-3 pre-miRNAs were identified in CyHV-1 and CyHV-2. This is the first report of miRNA and moRNA genes encoded by members of the Alloherpesviridae family, a group distantly related to the Herpesviridae family. The discovery of these novel CyHV-3 genes may help further our understanding of the biology of this economically important virus and their encoded miRNAs may have potential as biomarkers for the diagnosis of latent CyHV-3.
Description: Data Availability: Relevant data are contained within the paper and its Supporting Information files. All unique sequence tags obtained following virus infections are available for download via the Dryad Digital Repository (http://dx.doi.org/10.5061/dryad.3c7m4).
URI: http://hdl.handle.net/10793/1081
Appears in Collections:Peer Reviewed Scientific Papers

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